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Brilva Fluor™ 488 Rapid Labeling Kit
Product introduction
Brilva Fluor™ 488 Rapid Labeling Kit is a ready-to-use kit for covalent labeling of antibodies, recombinant proteins, enzymes and other biomolecules containing accessible primary amines. The NHS ester dye reacts under mildly alkaline conditions at room temperature in approximately 30 minutes, followed by ultrafiltration to remove unreacted free dye.
Features
- Rapid labeling — Approximately 30-minute conjugation reaction at room temperature.
- Simple workflow — Preconfigured labeling reagents and ultrafiltration-based removal of free dye.
- High-brightness fluorescence — Optimized for 488 nm / FITC-compatible fluorescence detection workflows.
- Flexible protein labeling — Supports antibodies and multiple compatible protein formats.
Product Overview
Brilva Fluor™ 488 Rapid Labeling Kit is a ready-to-use fluorescent protein labeling kit designed for covalent labeling of antibodies, recombinant proteins, enzymes and other biomolecules containing accessible primary amines.
The NHS ester dye reacts with primary amines on proteins under mildly alkaline conditions. The conjugation reaction can be completed at room temperature in approximately 30 minutes, followed by ultrafiltration to remove unreacted free dye.
- SKU: FP268004
- Product type: Fluorescent Protein Labeling Kit
- Technology: NHS Ester Fluorescent Labeling
- Standard specification: 5 reactions × 100 μg protein/reaction
Key Specifications
- Excitation maximum: 494 nm
- Emission maximum: 517 nm
- Molecular weight: ~643.4
- Extinction coefficient: 73,000 cm⁻¹M⁻¹
- CF at 280 nm: 0.11
- Brightness: High
- Recommended channel: 488 nm / FITC
- Confocal FITC group
Applications
Suitable for fluorescent labeling of:
- Antibodies
- Antibody fragments
- Recombinant proteins
- Enzymes
- Antigens
- Peptides and other compatible biomolecules
Typical downstream use may include fluorescence-based protein and antibody research.
Labeling Principle, Samples & Storage
Brilva Fluor labeling kits use NHS ester chemistry. The NHS ester reacts with accessible primary amines, primarily lysine residues and N-terminal amino groups, forming stable covalent amide bonds between the fluorescent dye and the target protein.
Preferred protein concentration: 0.5–4 mg/mL. Recommended target molecular weight: ≥10 kDa. Samples should be prepared in compatible buffers without interfering components.
Avoid incompatible components identified in the product instructions, including Tris, glycine and other interfering buffer components. All dye handling and labeling steps should be protected from light.
Dye-NHS component: −80°C, protected from light. Other kit components: follow the official product manual.
For Research Use Only (RUO). Not for diagnostic, therapeutic or human use.
User Manual
Download the user manual for kit components, workflow and handling instructions.
