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Brilva Fluor™ 647 Rapid Labeling Kit
Product introduction
Brilva Fluor™ 647 Rapid Labeling Kit is a ready-to-use kit for covalent labeling of antibodies, recombinant proteins, enzymes and other biomolecules containing accessible primary amines. The Brilva Fluor 647 NHS ester reacts under mildly alkaline conditions at room temperature in approximately 30 minutes, followed by ultrafiltration to remove unreacted free dye.
Features
- Rapid labeling — Approximately 30-minute conjugation reaction at room temperature.
- Broad protein compatibility — Supports antibodies and multiple compatible protein formats.
- Far-red fluorescence — Designed for 640 nm excitation and APC / Cy5-compatible detection workflows.
- Simple purification — Ultrafiltration removes unreacted fluorescent dye following conjugation.
Product Overview
Brilva Fluor™ 647 Rapid Labeling Kit is a ready-to-use fluorescent protein labeling kit for covalent labeling of antibodies, recombinant proteins, enzymes and other biomolecules containing accessible primary amines.
The Brilva Fluor 647 NHS ester reacts with protein primary amines under mildly alkaline conditions. Conjugation can be completed at room temperature in approximately 30 minutes, followed by ultrafiltration to remove unreacted free dye.
- SKU: FP268011
- Product type: Fluorescent Protein Labeling Kit
- Technology: NHS Ester Fluorescent Labeling
- Standard specification: 5 reactions × 100 μg protein/reaction
Key Specifications
- Excitation maximum: 651 nm
- Emission maximum: 672 nm
- Molecular weight: ~1250
- Extinction coefficient: 270,000 cm⁻¹M⁻¹
- CF at 280 nm: 0.03
- Brightness: High
- Recommended channel: 640 nm / APC
- Cy5 group
Applications
Suitable for fluorescent labeling of:
- Primary antibodies
- Secondary antibodies
- Antibody fragments
- Recombinant proteins
- Antigens
- Peptides
- Enzymes and other compatible proteins
Typical applications include immunofluorescence, flow cytometry, western blot and related fluorescence-based research workflows.
Labeling Principle, Samples & Storage
Brilva Fluor labeling kits use NHS ester chemistry. The NHS ester reacts with accessible primary amines, primarily lysine residues and N-terminal amino groups, forming stable covalent amide bonds between the fluorescent dye and the target protein.
Preferred protein concentration: 0.5–4 mg/mL. Recommended target molecular weight: ≥10 kDa. Samples should be prepared in compatible buffers without interfering components.
Avoid incompatible components identified in the product instructions, including Tris, glycine and other interfering buffer components. All dye handling and labeling steps should be protected from light.
Dye-NHS component: −80°C, protected from light. Other kit components: follow the official product manual.
For Research Use Only (RUO). Not for diagnostic, therapeutic or human use.
User Manual
Download the user manual for kit components, workflow and handling instructions.
